Molecular biologists routinely clone genetic constructs from DNA segments and formulate plans to assemble them. However, manual assembly planning is complex, error ...

Our ability to cut and paste DNA from different sources and to assemble it into gene constructs has been one of the key drivers of biological research and biotechnology over the past four decades.

Extracytoplasmic function sigma factors (ECFs) have been successfully used for constructing predictable artificial gene circuits bacteria like Escherichia coli, but their transferability between ...