STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to achieve an improvement in resolution, …

Jan 29, 2018 · Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical …

Stimulated Emission Depletion (STED) microscopy is a fluorescence microscopy super-resolution technique that is able to circumvent the optical diffraction limit. STED microscopy was first …

In STED microscopy, the specimen is illuminated by two synchronized ultrafast co-linear sources consisting of an excitation laser pulse followed by a red-shifted depletion laser pulse that is …

Jun 8, 2025 · In this series of articles on STED microscopy, I will cover more about how it works, optimising staining, and share some tips and tricks on how to get the most out of it.

STED microscopy uses two laser pulses to localize fluorescence at each focal spot. The first pulse is used to excite a fluorophore to its fluorescent state, and the second pulse is a …

Researchers have used STED to image the fine structures within neurons, including the distribution of synaptic proteins and the organization of synaptic vesicles, which are tiny sacs …

Stimulated emission depletion microscopy (STED) is a fluorescence microscopy technique that overcomes the diffraction limited resolution of confocal microscopes.

The STED donut determines where the fluorescence originates in space; therefore, imaging with a single STED line ensures that the different channels (colors) are intrinsically aligned.

The well-known saying of “Seeing is believing” became even more apt in biology when stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel laureate S. Hell …