What effect does vortexing have on a fluid sample that simple ...
A single vortex is essential for any "wet-lab" for the reason bobthejoe describes. The vortex not only provides a different profile of mixing, but has the power to mix viscous fluids that would otherwise …
PCR Mastermix Preparation - Biology Stack Exchange
Feb 5, 2019 · I am currently preparing a PCR mastermix for the first time, and have a few questions regarding the distribution. I am trying to amplify a specific gene cluster in a sample of DNA so I can …
Forgot to vortex antibody before staining - Biology Stack Exchange
Jun 27, 2016 · Ugh. Did an immunofluorescence experiment last weekend, forgot to vortex both my primary and my secondary antibody solutions. And my final result looks dimmer than it should be. Is …
Why is it advised to avoid bubble formation during mixing?
Feb 4, 2016 · I have been told not to vortex solution containing protein. The reason I was given is bubble formation. Here I am interested in the effect of bubble formation in general.
What is the optimal method to resuspend competent cells during wash ...
Aug 1, 2024 · Electrocompetent cells are a bit more finicky than chemically competent in this respect. Generally you do need to be gentle during the preparation - swirling is fine, but vortexing is not. Too …
DNA extraction from plants and algae don't use phenol. Why?
Oct 15, 2016 · Add 20ml of phenol:chloroform:isoamyl alcohol (PCI, 25:24:1). Cap the tube and mix vigorously by vortex or inversion. Centrifuge for 10 minutes at 13,000×g and 20-25°C to separate the …
molecular biology - How to pipette accurate volumes from BSA …
Nov 29, 2016 · 2 Never vortex the BSA solution. The best way to prepare a BSA solution is to put the BSA powder on the buffer solution (or water) instead of the other way round. Tap or rock the tube to …
biochemistry - Clarify pre-column pressure vs. system pressure vs ...
Dec 30, 2016 · Background: I am using the Äkta pure system for protein purification, with prepacked columns from GE-healthcare. Just to take one example, I use the HisTrap 5 mL column (IMAC: …
Storing Taqman plates with polymerase in fridge before running
May 10, 2015 · I stored several pre-mixed (with all the components, the polymerase included) plates in a -20˚C freezer for up to 1 month and still worked beautifully. Was using Taqman reagents. Would opt …
Why does hemagglutinin transfection fail in HEK293 cells?
Jan 20, 2023 · Resuspend in 1ml of ice-cold PBS Centrifuge at 400g at 4C for 5min, and aspirate the supernatant Resuspend in 100µl of RIPA buffer containing protease inhibitor cocktail Vortex for 1 …